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p s262 anti tau antibody  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank p s262 anti tau antibody
    a, WB and quantification showing effect of aging on <t>S262</t> phosphorylation of endogenous fly tau. b , WB and quantification showing effect of aging on endogenous mouse tau phosphorylation at S262, AT100, and PHF-1 sites. p-tau level is normalized with total tau or actin in a and b . c , WB and quantification of p-tau levels in WT mouse cortex of non-stressed control, heat-stressed, and heat-stressed plus CPT-treated animals, as well as the cortex of non-stressed control, heat-stressed, and heat-stress plus CPT-treated tau KO mice. d , e , Measurements of ROS and NAD + /NADH ratio of non-stressed or stressed WT mouse cortex cells, or mitochondria purified from these cells and respiring under RET ( d ) or FET ( e ) conditions. f , g , Measurements of ROS and NAD + /NADH ratio of non-stressed or heat-stressed WT Drosophila brain cells or mitochondria purified from these cells and respiring under RET ( f ) or FET ( g ) conditions. h , WB analysis of heat stress-induced p-S262 tau level in WT and tau KO fly brain. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, c ), or two-tailed unpaired Student’s t test ( b, d, e, f, g ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For fly studies, n=3-5 biological repeats; For mouse studies, each data point represents an individual mouse.
    P S262 Anti Tau Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5a6+anti+tau/anti-Tau/bio_rxiv__64898__2026__04__04__716514-561-35-44
    Average 95 stars, based on 21 article reviews
    p s262 anti tau antibody - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Tau-induced mitochondrial reverse electron transport drives neurodegeneration"

    Article Title: Tau-induced mitochondrial reverse electron transport drives neurodegeneration

    Journal: bioRxiv

    doi: 10.64898/2026.04.04.716514

    a, WB and quantification showing effect of aging on S262 phosphorylation of endogenous fly tau. b , WB and quantification showing effect of aging on endogenous mouse tau phosphorylation at S262, AT100, and PHF-1 sites. p-tau level is normalized with total tau or actin in a and b . c , WB and quantification of p-tau levels in WT mouse cortex of non-stressed control, heat-stressed, and heat-stressed plus CPT-treated animals, as well as the cortex of non-stressed control, heat-stressed, and heat-stress plus CPT-treated tau KO mice. d , e , Measurements of ROS and NAD + /NADH ratio of non-stressed or stressed WT mouse cortex cells, or mitochondria purified from these cells and respiring under RET ( d ) or FET ( e ) conditions. f , g , Measurements of ROS and NAD + /NADH ratio of non-stressed or heat-stressed WT Drosophila brain cells or mitochondria purified from these cells and respiring under RET ( f ) or FET ( g ) conditions. h , WB analysis of heat stress-induced p-S262 tau level in WT and tau KO fly brain. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, c ), or two-tailed unpaired Student’s t test ( b, d, e, f, g ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For fly studies, n=3-5 biological repeats; For mouse studies, each data point represents an individual mouse.
    Figure Legend Snippet: a, WB and quantification showing effect of aging on S262 phosphorylation of endogenous fly tau. b , WB and quantification showing effect of aging on endogenous mouse tau phosphorylation at S262, AT100, and PHF-1 sites. p-tau level is normalized with total tau or actin in a and b . c , WB and quantification of p-tau levels in WT mouse cortex of non-stressed control, heat-stressed, and heat-stressed plus CPT-treated animals, as well as the cortex of non-stressed control, heat-stressed, and heat-stress plus CPT-treated tau KO mice. d , e , Measurements of ROS and NAD + /NADH ratio of non-stressed or stressed WT mouse cortex cells, or mitochondria purified from these cells and respiring under RET ( d ) or FET ( e ) conditions. f , g , Measurements of ROS and NAD + /NADH ratio of non-stressed or heat-stressed WT Drosophila brain cells or mitochondria purified from these cells and respiring under RET ( f ) or FET ( g ) conditions. h , WB analysis of heat stress-induced p-S262 tau level in WT and tau KO fly brain. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, c ), or two-tailed unpaired Student’s t test ( b, d, e, f, g ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For fly studies, n=3-5 biological repeats; For mouse studies, each data point represents an individual mouse.

    Techniques Used: Phospho-proteomics, Control, Purification, Two Tailed Test

    a, climbing activity assay of control flies and neuronal tau-R406W expressing flies with or without CPT treatment at different ages. b , Survival curve of control flies and neuronal tau-R406W expression flies with or without CPT treatment. c , Aversive taste memory assay of control flies and neuronal tau-R406W flies with or without CPT treatment. d , e , Immunostaining ( d ) and quantification ( e ) of signal intensities of TH-positive PPL1 cluster dopaminergic neurons and p62 and ubiquitin (ubi) positive protein aggregates in the brains of control flies and neuronal tau-R406W flies with or without CPT treatment. f , g , WB and quantification showing effect of CPT on p-S262 ( f ) and PHF-1 p-tau ( g ) level in pan-neuronal elav-Gal4 > ( f ) or photoreceptor neuron-specific GMR-Gal4 > ( g ) tau-R406W expression flies. h - j , Effect of RET inhibition by NDUFS3 RNAi on climbing activity ( h ), survival ( i ), and aversive taste memory ( j ) of pan-neuronal tau-R406W expression flies. k , WB and quantification showing effect of NDUFS3 RNAi on p-tau levels in pan-neuronal tau-R406W flies. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, e, f, g, h ), group analysis using multiple t test with Sidak-Bonferroni multiple comparison ( c , j ), two-tailed unpaired Student’s t test ( k ), or Kaplan Meier survival analysis ( b, i ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. Each data point represents independent experimental repeat.
    Figure Legend Snippet: a, climbing activity assay of control flies and neuronal tau-R406W expressing flies with or without CPT treatment at different ages. b , Survival curve of control flies and neuronal tau-R406W expression flies with or without CPT treatment. c , Aversive taste memory assay of control flies and neuronal tau-R406W flies with or without CPT treatment. d , e , Immunostaining ( d ) and quantification ( e ) of signal intensities of TH-positive PPL1 cluster dopaminergic neurons and p62 and ubiquitin (ubi) positive protein aggregates in the brains of control flies and neuronal tau-R406W flies with or without CPT treatment. f , g , WB and quantification showing effect of CPT on p-S262 ( f ) and PHF-1 p-tau ( g ) level in pan-neuronal elav-Gal4 > ( f ) or photoreceptor neuron-specific GMR-Gal4 > ( g ) tau-R406W expression flies. h - j , Effect of RET inhibition by NDUFS3 RNAi on climbing activity ( h ), survival ( i ), and aversive taste memory ( j ) of pan-neuronal tau-R406W expression flies. k , WB and quantification showing effect of NDUFS3 RNAi on p-tau levels in pan-neuronal tau-R406W flies. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, e, f, g, h ), group analysis using multiple t test with Sidak-Bonferroni multiple comparison ( c , j ), two-tailed unpaired Student’s t test ( k ), or Kaplan Meier survival analysis ( b, i ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. Each data point represents independent experimental repeat.

    Techniques Used: Activity Assay, Control, Expressing, Immunostaining, Ubiquitin Proteomics, Inhibition, Comparison, Two Tailed Test

    a, b , Representative MRI images ( a ) and data quantification ( b ) of olfactory bulb (OB) volume. c-e , Representative images and data quantification of DNA-damage marker γH2AX ( c ), apoptosis marker cleaved caspase 3 ( d ), and tau aggregation as indicated by ThioS staining ( e ) in brain sections of rTg4510 mice treated with vehicle or CPT. f , g , Representative images ( f ) and quantification ( g ) of PHF-1 and CP13 immunostaining in the hippocampal CA1 and cortex regions of rTg4510 mouse brain. h, i , Immunoblots ( h ) and data quantification ( i ) of p-tau (PHF-1, CP13, AT100, p-T231, p-S262), total tau (HT7), and NDUFS3 levels in the cortex of 8-month-old rTg4510 mice. p-tau levels were normalized with total tau. All data are means ± SEM; statistical significance was determined by two-tailed unpaired Student’s t test; NDUFS3/actin in i was quantified by one-way ANOVA with Tukey’s post hoc test. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001 ( b : n=4 mice/group; c-e , g : n=5 mice/group, each data point represents an average of 6 images/mouse; i : each data point represents an individual mouse).
    Figure Legend Snippet: a, b , Representative MRI images ( a ) and data quantification ( b ) of olfactory bulb (OB) volume. c-e , Representative images and data quantification of DNA-damage marker γH2AX ( c ), apoptosis marker cleaved caspase 3 ( d ), and tau aggregation as indicated by ThioS staining ( e ) in brain sections of rTg4510 mice treated with vehicle or CPT. f , g , Representative images ( f ) and quantification ( g ) of PHF-1 and CP13 immunostaining in the hippocampal CA1 and cortex regions of rTg4510 mouse brain. h, i , Immunoblots ( h ) and data quantification ( i ) of p-tau (PHF-1, CP13, AT100, p-T231, p-S262), total tau (HT7), and NDUFS3 levels in the cortex of 8-month-old rTg4510 mice. p-tau levels were normalized with total tau. All data are means ± SEM; statistical significance was determined by two-tailed unpaired Student’s t test; NDUFS3/actin in i was quantified by one-way ANOVA with Tukey’s post hoc test. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001 ( b : n=4 mice/group; c-e , g : n=5 mice/group, each data point represents an average of 6 images/mouse; i : each data point represents an individual mouse).

    Techniques Used: Olfactory, Marker, Staining, Immunostaining, Western Blot, Two Tailed Test

    a , Effect of H 2 O 2 and FK866 on the viability of tau-P301L hiPSC neurons and isogenic wildtype controls after 24h treatment, and the rescuing effect of CPT. b , Effect of H 2 O 2 and FK866 co-treatment on the viability of control and tau KD hiPSC neurons and the rescuing effect of CPT. c, d , Effect of DES treatment on the viability of control and tau KD hiPSC neurons ( c ) and the rescuing effect of CPT in DES (20 mM) treated control hiPSC neurons ( d ). e - g , WB and quantification showing the effect of inhibiting VDAC, Hsp70, or Hsp90 on PHF-1 tau entry into mitochondria without affecting total PHF-1 tau levels ( e ), and quantification of the effect of inhibitor treatment on RET activity ( f ) and stress sensitivity ( g ) of APP hiPSC neurons. h , Measurement of RET ROS and NAD + /NADH in purified mitochondria from control and tau-WT-EGFP or tau-S2A-EGFP-transfected normal hiPSC neurons. i , Representative images and quantification of p-S262 tau in tau-WT-EGFP or tau-S2A-EGFP transfected control hiPSC neurons with or without DES treatment. j , Measurement of RET ROS and NAD + /NADH in purified mitochondria from EGFP or MKI-EGFP transduced APP hiPSC neurons. k , Representative images and quantification of p-S262 tau in EGFP or MKI-EGFP transfected APP hiPSC neurons. l, m , Immunoblots ( l ) and quantification ( m ) showing effects of the various treatments on normalized levels of p-tau species in APP hiPSC neurons. n, o , Immunoblots ( n ) and quantification ( o ) showing effect of DES or DES/CPT co-treatment on normalized levels of p-tau species in control hiPSC neurons. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, b, d, g, h, i, o ), two-way ANOVA with Tukey’s post hoc test ( c ), two-tailed unpaired Student’s t test ( j, k ), or one-way ANOVA with Dunnett’s multiple test ( e, f , m ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001 ( a, b, c, d, g : n=6/group from 3 biological replicates and 2 wells/experiment; e, f, h, j, m, o : n=3 biological replicates; i, k : n=3 biological replicates, and each data point represents an average of 6 cells/experiment).
    Figure Legend Snippet: a , Effect of H 2 O 2 and FK866 on the viability of tau-P301L hiPSC neurons and isogenic wildtype controls after 24h treatment, and the rescuing effect of CPT. b , Effect of H 2 O 2 and FK866 co-treatment on the viability of control and tau KD hiPSC neurons and the rescuing effect of CPT. c, d , Effect of DES treatment on the viability of control and tau KD hiPSC neurons ( c ) and the rescuing effect of CPT in DES (20 mM) treated control hiPSC neurons ( d ). e - g , WB and quantification showing the effect of inhibiting VDAC, Hsp70, or Hsp90 on PHF-1 tau entry into mitochondria without affecting total PHF-1 tau levels ( e ), and quantification of the effect of inhibitor treatment on RET activity ( f ) and stress sensitivity ( g ) of APP hiPSC neurons. h , Measurement of RET ROS and NAD + /NADH in purified mitochondria from control and tau-WT-EGFP or tau-S2A-EGFP-transfected normal hiPSC neurons. i , Representative images and quantification of p-S262 tau in tau-WT-EGFP or tau-S2A-EGFP transfected control hiPSC neurons with or without DES treatment. j , Measurement of RET ROS and NAD + /NADH in purified mitochondria from EGFP or MKI-EGFP transduced APP hiPSC neurons. k , Representative images and quantification of p-S262 tau in EGFP or MKI-EGFP transfected APP hiPSC neurons. l, m , Immunoblots ( l ) and quantification ( m ) showing effects of the various treatments on normalized levels of p-tau species in APP hiPSC neurons. n, o , Immunoblots ( n ) and quantification ( o ) showing effect of DES or DES/CPT co-treatment on normalized levels of p-tau species in control hiPSC neurons. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, b, d, g, h, i, o ), two-way ANOVA with Tukey’s post hoc test ( c ), two-tailed unpaired Student’s t test ( j, k ), or one-way ANOVA with Dunnett’s multiple test ( e, f , m ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001 ( a, b, c, d, g : n=6/group from 3 biological replicates and 2 wells/experiment; e, f, h, j, m, o : n=3 biological replicates; i, k : n=3 biological replicates, and each data point represents an average of 6 cells/experiment).

    Techniques Used: Control, Activity Assay, Purification, Transfection, Western Blot, Two Tailed Test

    Related Articles

    Incubation:

    Article Title: Tau and Futsch play non-neuronal roles in mediating morphogenesis and function of Drosophila Malpighian tubules
    Article Snippet: The Secondary antibodies used are as follows: Goat anti-mouse Alexa Fluor 488 (Jackson ImmunoResearch, 1:100), goat anti-mouse Alexa Fluor 546 (Invitrogen, 1:200). .. Membranes were then incubated overnight at 4°C with primary antibodies diluted in blocking solution: 5A6 Anti-tau (DSHB, 1:20, Cat # 528487 ), p1D9 Anti-Rho1 (DSHB, 1:20, Cat # 528263 ), and 2G3-3D7 Anti-Actinin (DSHB,1:1000, Cat # 2721943 ). .. After primary antibody incubation, membranes were washed three times in 0.1% TBST (15 minutes each wash at room temperature), followed by incubation with HRP-conjugated anti-mouse secondary antibody (Invitrogen, 1:1000, Catalog # 31430) for 2 hours at room temperature.

    Blocking Assay:

    Article Title: Tau and Futsch play non-neuronal roles in mediating morphogenesis and function of Drosophila Malpighian tubules
    Article Snippet: The Secondary antibodies used are as follows: Goat anti-mouse Alexa Fluor 488 (Jackson ImmunoResearch, 1:100), goat anti-mouse Alexa Fluor 546 (Invitrogen, 1:200). .. Membranes were then incubated overnight at 4°C with primary antibodies diluted in blocking solution: 5A6 Anti-tau (DSHB, 1:20, Cat # 528487 ), p1D9 Anti-Rho1 (DSHB, 1:20, Cat # 528263 ), and 2G3-3D7 Anti-Actinin (DSHB,1:1000, Cat # 2721943 ). .. After primary antibody incubation, membranes were washed three times in 0.1% TBST (15 minutes each wash at room temperature), followed by incubation with HRP-conjugated anti-mouse secondary antibody (Invitrogen, 1:1000, Catalog # 31430) for 2 hours at room temperature.

    other:

    Article Title: Tau and Futsch play non-neuronal roles in mediating morphogenesis and function of Drosophila Malpighian tubules
    Article Snippet: Anti-Ct (DSHB, 1:10, Cat # 528186 ), Anti-Dlg (DSHB, 1:20, Cat # 528203 ), p1D9 Anti-Rho1 (DSHB, 1:20, Cat # 528263 ), a5 Na+/K+ ATPase (DSHB, 1:20, Cat # 2166869 ), E7 Anti-β tubulin (DSHB, 1:200, Cat # 2315513 ), 5A6 Anti-tau (DSHB, 1:20, Cat # 528487 ), Anti-22C10 (DSHB, 1:50, Cat # 528403 ), shg D-Cadherin (DSHB, 1:50, Cat # 528120 ) .

    Article Title: Tau and Futsch play non-neuronal roles in mediating morphogenesis and function of Drosophila Malpighian tubules
    Article Snippet: For imaging purposes, the samples were mounted in DABCO antifadant (Sigma, Cat# D27802).



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    95
    Developmental Studies Hybridoma Bank mouse 5a6 anti tau
    a, WB and quantification showing effect of aging on <t>S262</t> phosphorylation of endogenous fly tau. b , WB and quantification showing effect of aging on endogenous mouse tau phosphorylation at S262, AT100, and PHF-1 sites. p-tau level is normalized with total tau or actin in a and b . c , WB and quantification of p-tau levels in WT mouse cortex of non-stressed control, heat-stressed, and heat-stressed plus CPT-treated animals, as well as the cortex of non-stressed control, heat-stressed, and heat-stress plus CPT-treated tau KO mice. d , e , Measurements of ROS and NAD + /NADH ratio of non-stressed or stressed WT mouse cortex cells, or mitochondria purified from these cells and respiring under RET ( d ) or FET ( e ) conditions. f , g , Measurements of ROS and NAD + /NADH ratio of non-stressed or heat-stressed WT Drosophila brain cells or mitochondria purified from these cells and respiring under RET ( f ) or FET ( g ) conditions. h , WB analysis of heat stress-induced p-S262 tau level in WT and tau KO fly brain. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, c ), or two-tailed unpaired Student’s t test ( b, d, e, f, g ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For fly studies, n=3-5 biological repeats; For mouse studies, each data point represents an individual mouse.
    Mouse 5a6 Anti Tau, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a, WB and quantification showing effect of aging on S262 phosphorylation of endogenous fly tau. b , WB and quantification showing effect of aging on endogenous mouse tau phosphorylation at S262, AT100, and PHF-1 sites. p-tau level is normalized with total tau or actin in a and b . c , WB and quantification of p-tau levels in WT mouse cortex of non-stressed control, heat-stressed, and heat-stressed plus CPT-treated animals, as well as the cortex of non-stressed control, heat-stressed, and heat-stress plus CPT-treated tau KO mice. d , e , Measurements of ROS and NAD + /NADH ratio of non-stressed or stressed WT mouse cortex cells, or mitochondria purified from these cells and respiring under RET ( d ) or FET ( e ) conditions. f , g , Measurements of ROS and NAD + /NADH ratio of non-stressed or heat-stressed WT Drosophila brain cells or mitochondria purified from these cells and respiring under RET ( f ) or FET ( g ) conditions. h , WB analysis of heat stress-induced p-S262 tau level in WT and tau KO fly brain. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, c ), or two-tailed unpaired Student’s t test ( b, d, e, f, g ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For fly studies, n=3-5 biological repeats; For mouse studies, each data point represents an individual mouse.

    Journal: bioRxiv

    Article Title: Tau-induced mitochondrial reverse electron transport drives neurodegeneration

    doi: 10.64898/2026.04.04.716514

    Figure Lengend Snippet: a, WB and quantification showing effect of aging on S262 phosphorylation of endogenous fly tau. b , WB and quantification showing effect of aging on endogenous mouse tau phosphorylation at S262, AT100, and PHF-1 sites. p-tau level is normalized with total tau or actin in a and b . c , WB and quantification of p-tau levels in WT mouse cortex of non-stressed control, heat-stressed, and heat-stressed plus CPT-treated animals, as well as the cortex of non-stressed control, heat-stressed, and heat-stress plus CPT-treated tau KO mice. d , e , Measurements of ROS and NAD + /NADH ratio of non-stressed or stressed WT mouse cortex cells, or mitochondria purified from these cells and respiring under RET ( d ) or FET ( e ) conditions. f , g , Measurements of ROS and NAD + /NADH ratio of non-stressed or heat-stressed WT Drosophila brain cells or mitochondria purified from these cells and respiring under RET ( f ) or FET ( g ) conditions. h , WB analysis of heat stress-induced p-S262 tau level in WT and tau KO fly brain. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, c ), or two-tailed unpaired Student’s t test ( b, d, e, f, g ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For fly studies, n=3-5 biological repeats; For mouse studies, each data point represents an individual mouse.

    Article Snippet: The following primary antibodies and dilutions were used: mouse anti-tau PHF1, 1:500 (Gift of Peter Davies); mouse anti-tau CP13, 1:500 (Gift of Peter Davies); rat anti-dSDHA and rabbit anti-dNDUFV1 1:1000 (Gift from Dr. Edward Owusu-Ansah); p-S262 anti-tau antibody 1:500 (ThermoFisher #44-750G); mouse anti-dTau 1:500 (DSHB #5A6); rabbit anti-Tom20 1:1000 (abclonal #A19403); rabbit anti-cytochrome c 1:1000 (Proteintech #10993-1-AP); rabbit anti-LonP1 1:1000 (Proteintech #15440-1-AP); rabbit anti-Map2 1:1000 (Proteintech #17490-1-AP); rabbit anti-NDUFS1 1:1000 (Proteintech #12444-1-AP); rabbit anti-NDUFA10 1:1000 (Proteintech #32947-1-AP); rabbit anti-SDHA 1:1000 (Proteintech #14865-1-AP), rabbit anti-ATP5α 1:1000 (abcam #ab14748); mouse anti-tau AT100, 1:500 (Invitrogen, MN1060); mouse anti-tau HT7, 1:1000 (Invitrogen, MN1000); mouse anti-Tau7, 1:1000 (Sigma, MAB2239); rabbit anti-tau Thr231, 1:500 (Invitrogen, 44-746G); rabbit anti-NDUFV1, 1:1000 (Proteintech, 11238-1-AP); rabbit anti-NDUFS3, 1:1000 (Proteintech, 15066-1-AP); rabbit anti-GFAP, 1:500 (Proteintech, 16825-1-AP); rabbit anti-IL-1β, 1:500 (Proteintech, 16806-1-AP); rabbit anti-Cleaved Caspase-1, 1:500 (Cell Signaling Technology, 4199T); rabbit anti-ASC/TMS1, 1:500 (Cell Signaling Technology, 67824T); rabbit anti-Drp1, 1:500 (Cell Signaling Technology, 4494S); rabbit anti-Opa1, 1:500 (Cell Signaling Technology, 80471T); rabbit anti-Glu2B, 1:500 (Cell Signaling Technology, 4212T); rabbit anti-p-GSK3β, 1:500 (Cell Signaling Technology, 5558T); rabbit anti-GSK3β, 1:500 (Cell Signaling Technology, 12456S); rabbit anti-MARK4, 1:500 (Cell Signaling Technology, 4834S); rabbit anti-MFN2, 1:500 (ProSci, 7863); mouse anti-PSD95, 1:500 (BioLegend, 810302); mouse anti-PP2A, 1:500 (BD Biosciences, 610556); mouse anti-Tau46, 1:500 (Sigma, T9450); rabbit anti-p-PAR-1(T408) , 1:500; rabbit anti-VDAC1, 1:500 (Proteintech, 55259-1-AP); rabbit anti-Hsp70, 1:200 (Santa Cruze, sc-25837); rabbit anti-Hsp90, 1:500 (Proteintech, 13171-1-AP).

    Techniques: Phospho-proteomics, Control, Purification, Two Tailed Test

    a, climbing activity assay of control flies and neuronal tau-R406W expressing flies with or without CPT treatment at different ages. b , Survival curve of control flies and neuronal tau-R406W expression flies with or without CPT treatment. c , Aversive taste memory assay of control flies and neuronal tau-R406W flies with or without CPT treatment. d , e , Immunostaining ( d ) and quantification ( e ) of signal intensities of TH-positive PPL1 cluster dopaminergic neurons and p62 and ubiquitin (ubi) positive protein aggregates in the brains of control flies and neuronal tau-R406W flies with or without CPT treatment. f , g , WB and quantification showing effect of CPT on p-S262 ( f ) and PHF-1 p-tau ( g ) level in pan-neuronal elav-Gal4 > ( f ) or photoreceptor neuron-specific GMR-Gal4 > ( g ) tau-R406W expression flies. h - j , Effect of RET inhibition by NDUFS3 RNAi on climbing activity ( h ), survival ( i ), and aversive taste memory ( j ) of pan-neuronal tau-R406W expression flies. k , WB and quantification showing effect of NDUFS3 RNAi on p-tau levels in pan-neuronal tau-R406W flies. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, e, f, g, h ), group analysis using multiple t test with Sidak-Bonferroni multiple comparison ( c , j ), two-tailed unpaired Student’s t test ( k ), or Kaplan Meier survival analysis ( b, i ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. Each data point represents independent experimental repeat.

    Journal: bioRxiv

    Article Title: Tau-induced mitochondrial reverse electron transport drives neurodegeneration

    doi: 10.64898/2026.04.04.716514

    Figure Lengend Snippet: a, climbing activity assay of control flies and neuronal tau-R406W expressing flies with or without CPT treatment at different ages. b , Survival curve of control flies and neuronal tau-R406W expression flies with or without CPT treatment. c , Aversive taste memory assay of control flies and neuronal tau-R406W flies with or without CPT treatment. d , e , Immunostaining ( d ) and quantification ( e ) of signal intensities of TH-positive PPL1 cluster dopaminergic neurons and p62 and ubiquitin (ubi) positive protein aggregates in the brains of control flies and neuronal tau-R406W flies with or without CPT treatment. f , g , WB and quantification showing effect of CPT on p-S262 ( f ) and PHF-1 p-tau ( g ) level in pan-neuronal elav-Gal4 > ( f ) or photoreceptor neuron-specific GMR-Gal4 > ( g ) tau-R406W expression flies. h - j , Effect of RET inhibition by NDUFS3 RNAi on climbing activity ( h ), survival ( i ), and aversive taste memory ( j ) of pan-neuronal tau-R406W expression flies. k , WB and quantification showing effect of NDUFS3 RNAi on p-tau levels in pan-neuronal tau-R406W flies. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, e, f, g, h ), group analysis using multiple t test with Sidak-Bonferroni multiple comparison ( c , j ), two-tailed unpaired Student’s t test ( k ), or Kaplan Meier survival analysis ( b, i ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. Each data point represents independent experimental repeat.

    Article Snippet: The following primary antibodies and dilutions were used: mouse anti-tau PHF1, 1:500 (Gift of Peter Davies); mouse anti-tau CP13, 1:500 (Gift of Peter Davies); rat anti-dSDHA and rabbit anti-dNDUFV1 1:1000 (Gift from Dr. Edward Owusu-Ansah); p-S262 anti-tau antibody 1:500 (ThermoFisher #44-750G); mouse anti-dTau 1:500 (DSHB #5A6); rabbit anti-Tom20 1:1000 (abclonal #A19403); rabbit anti-cytochrome c 1:1000 (Proteintech #10993-1-AP); rabbit anti-LonP1 1:1000 (Proteintech #15440-1-AP); rabbit anti-Map2 1:1000 (Proteintech #17490-1-AP); rabbit anti-NDUFS1 1:1000 (Proteintech #12444-1-AP); rabbit anti-NDUFA10 1:1000 (Proteintech #32947-1-AP); rabbit anti-SDHA 1:1000 (Proteintech #14865-1-AP), rabbit anti-ATP5α 1:1000 (abcam #ab14748); mouse anti-tau AT100, 1:500 (Invitrogen, MN1060); mouse anti-tau HT7, 1:1000 (Invitrogen, MN1000); mouse anti-Tau7, 1:1000 (Sigma, MAB2239); rabbit anti-tau Thr231, 1:500 (Invitrogen, 44-746G); rabbit anti-NDUFV1, 1:1000 (Proteintech, 11238-1-AP); rabbit anti-NDUFS3, 1:1000 (Proteintech, 15066-1-AP); rabbit anti-GFAP, 1:500 (Proteintech, 16825-1-AP); rabbit anti-IL-1β, 1:500 (Proteintech, 16806-1-AP); rabbit anti-Cleaved Caspase-1, 1:500 (Cell Signaling Technology, 4199T); rabbit anti-ASC/TMS1, 1:500 (Cell Signaling Technology, 67824T); rabbit anti-Drp1, 1:500 (Cell Signaling Technology, 4494S); rabbit anti-Opa1, 1:500 (Cell Signaling Technology, 80471T); rabbit anti-Glu2B, 1:500 (Cell Signaling Technology, 4212T); rabbit anti-p-GSK3β, 1:500 (Cell Signaling Technology, 5558T); rabbit anti-GSK3β, 1:500 (Cell Signaling Technology, 12456S); rabbit anti-MARK4, 1:500 (Cell Signaling Technology, 4834S); rabbit anti-MFN2, 1:500 (ProSci, 7863); mouse anti-PSD95, 1:500 (BioLegend, 810302); mouse anti-PP2A, 1:500 (BD Biosciences, 610556); mouse anti-Tau46, 1:500 (Sigma, T9450); rabbit anti-p-PAR-1(T408) , 1:500; rabbit anti-VDAC1, 1:500 (Proteintech, 55259-1-AP); rabbit anti-Hsp70, 1:200 (Santa Cruze, sc-25837); rabbit anti-Hsp90, 1:500 (Proteintech, 13171-1-AP).

    Techniques: Activity Assay, Control, Expressing, Immunostaining, Ubiquitin Proteomics, Inhibition, Comparison, Two Tailed Test

    a, b , Representative MRI images ( a ) and data quantification ( b ) of olfactory bulb (OB) volume. c-e , Representative images and data quantification of DNA-damage marker γH2AX ( c ), apoptosis marker cleaved caspase 3 ( d ), and tau aggregation as indicated by ThioS staining ( e ) in brain sections of rTg4510 mice treated with vehicle or CPT. f , g , Representative images ( f ) and quantification ( g ) of PHF-1 and CP13 immunostaining in the hippocampal CA1 and cortex regions of rTg4510 mouse brain. h, i , Immunoblots ( h ) and data quantification ( i ) of p-tau (PHF-1, CP13, AT100, p-T231, p-S262), total tau (HT7), and NDUFS3 levels in the cortex of 8-month-old rTg4510 mice. p-tau levels were normalized with total tau. All data are means ± SEM; statistical significance was determined by two-tailed unpaired Student’s t test; NDUFS3/actin in i was quantified by one-way ANOVA with Tukey’s post hoc test. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001 ( b : n=4 mice/group; c-e , g : n=5 mice/group, each data point represents an average of 6 images/mouse; i : each data point represents an individual mouse).

    Journal: bioRxiv

    Article Title: Tau-induced mitochondrial reverse electron transport drives neurodegeneration

    doi: 10.64898/2026.04.04.716514

    Figure Lengend Snippet: a, b , Representative MRI images ( a ) and data quantification ( b ) of olfactory bulb (OB) volume. c-e , Representative images and data quantification of DNA-damage marker γH2AX ( c ), apoptosis marker cleaved caspase 3 ( d ), and tau aggregation as indicated by ThioS staining ( e ) in brain sections of rTg4510 mice treated with vehicle or CPT. f , g , Representative images ( f ) and quantification ( g ) of PHF-1 and CP13 immunostaining in the hippocampal CA1 and cortex regions of rTg4510 mouse brain. h, i , Immunoblots ( h ) and data quantification ( i ) of p-tau (PHF-1, CP13, AT100, p-T231, p-S262), total tau (HT7), and NDUFS3 levels in the cortex of 8-month-old rTg4510 mice. p-tau levels were normalized with total tau. All data are means ± SEM; statistical significance was determined by two-tailed unpaired Student’s t test; NDUFS3/actin in i was quantified by one-way ANOVA with Tukey’s post hoc test. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001 ( b : n=4 mice/group; c-e , g : n=5 mice/group, each data point represents an average of 6 images/mouse; i : each data point represents an individual mouse).

    Article Snippet: The following primary antibodies and dilutions were used: mouse anti-tau PHF1, 1:500 (Gift of Peter Davies); mouse anti-tau CP13, 1:500 (Gift of Peter Davies); rat anti-dSDHA and rabbit anti-dNDUFV1 1:1000 (Gift from Dr. Edward Owusu-Ansah); p-S262 anti-tau antibody 1:500 (ThermoFisher #44-750G); mouse anti-dTau 1:500 (DSHB #5A6); rabbit anti-Tom20 1:1000 (abclonal #A19403); rabbit anti-cytochrome c 1:1000 (Proteintech #10993-1-AP); rabbit anti-LonP1 1:1000 (Proteintech #15440-1-AP); rabbit anti-Map2 1:1000 (Proteintech #17490-1-AP); rabbit anti-NDUFS1 1:1000 (Proteintech #12444-1-AP); rabbit anti-NDUFA10 1:1000 (Proteintech #32947-1-AP); rabbit anti-SDHA 1:1000 (Proteintech #14865-1-AP), rabbit anti-ATP5α 1:1000 (abcam #ab14748); mouse anti-tau AT100, 1:500 (Invitrogen, MN1060); mouse anti-tau HT7, 1:1000 (Invitrogen, MN1000); mouse anti-Tau7, 1:1000 (Sigma, MAB2239); rabbit anti-tau Thr231, 1:500 (Invitrogen, 44-746G); rabbit anti-NDUFV1, 1:1000 (Proteintech, 11238-1-AP); rabbit anti-NDUFS3, 1:1000 (Proteintech, 15066-1-AP); rabbit anti-GFAP, 1:500 (Proteintech, 16825-1-AP); rabbit anti-IL-1β, 1:500 (Proteintech, 16806-1-AP); rabbit anti-Cleaved Caspase-1, 1:500 (Cell Signaling Technology, 4199T); rabbit anti-ASC/TMS1, 1:500 (Cell Signaling Technology, 67824T); rabbit anti-Drp1, 1:500 (Cell Signaling Technology, 4494S); rabbit anti-Opa1, 1:500 (Cell Signaling Technology, 80471T); rabbit anti-Glu2B, 1:500 (Cell Signaling Technology, 4212T); rabbit anti-p-GSK3β, 1:500 (Cell Signaling Technology, 5558T); rabbit anti-GSK3β, 1:500 (Cell Signaling Technology, 12456S); rabbit anti-MARK4, 1:500 (Cell Signaling Technology, 4834S); rabbit anti-MFN2, 1:500 (ProSci, 7863); mouse anti-PSD95, 1:500 (BioLegend, 810302); mouse anti-PP2A, 1:500 (BD Biosciences, 610556); mouse anti-Tau46, 1:500 (Sigma, T9450); rabbit anti-p-PAR-1(T408) , 1:500; rabbit anti-VDAC1, 1:500 (Proteintech, 55259-1-AP); rabbit anti-Hsp70, 1:200 (Santa Cruze, sc-25837); rabbit anti-Hsp90, 1:500 (Proteintech, 13171-1-AP).

    Techniques: Olfactory, Marker, Staining, Immunostaining, Western Blot, Two Tailed Test

    a , Effect of H 2 O 2 and FK866 on the viability of tau-P301L hiPSC neurons and isogenic wildtype controls after 24h treatment, and the rescuing effect of CPT. b , Effect of H 2 O 2 and FK866 co-treatment on the viability of control and tau KD hiPSC neurons and the rescuing effect of CPT. c, d , Effect of DES treatment on the viability of control and tau KD hiPSC neurons ( c ) and the rescuing effect of CPT in DES (20 mM) treated control hiPSC neurons ( d ). e - g , WB and quantification showing the effect of inhibiting VDAC, Hsp70, or Hsp90 on PHF-1 tau entry into mitochondria without affecting total PHF-1 tau levels ( e ), and quantification of the effect of inhibitor treatment on RET activity ( f ) and stress sensitivity ( g ) of APP hiPSC neurons. h , Measurement of RET ROS and NAD + /NADH in purified mitochondria from control and tau-WT-EGFP or tau-S2A-EGFP-transfected normal hiPSC neurons. i , Representative images and quantification of p-S262 tau in tau-WT-EGFP or tau-S2A-EGFP transfected control hiPSC neurons with or without DES treatment. j , Measurement of RET ROS and NAD + /NADH in purified mitochondria from EGFP or MKI-EGFP transduced APP hiPSC neurons. k , Representative images and quantification of p-S262 tau in EGFP or MKI-EGFP transfected APP hiPSC neurons. l, m , Immunoblots ( l ) and quantification ( m ) showing effects of the various treatments on normalized levels of p-tau species in APP hiPSC neurons. n, o , Immunoblots ( n ) and quantification ( o ) showing effect of DES or DES/CPT co-treatment on normalized levels of p-tau species in control hiPSC neurons. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, b, d, g, h, i, o ), two-way ANOVA with Tukey’s post hoc test ( c ), two-tailed unpaired Student’s t test ( j, k ), or one-way ANOVA with Dunnett’s multiple test ( e, f , m ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001 ( a, b, c, d, g : n=6/group from 3 biological replicates and 2 wells/experiment; e, f, h, j, m, o : n=3 biological replicates; i, k : n=3 biological replicates, and each data point represents an average of 6 cells/experiment).

    Journal: bioRxiv

    Article Title: Tau-induced mitochondrial reverse electron transport drives neurodegeneration

    doi: 10.64898/2026.04.04.716514

    Figure Lengend Snippet: a , Effect of H 2 O 2 and FK866 on the viability of tau-P301L hiPSC neurons and isogenic wildtype controls after 24h treatment, and the rescuing effect of CPT. b , Effect of H 2 O 2 and FK866 co-treatment on the viability of control and tau KD hiPSC neurons and the rescuing effect of CPT. c, d , Effect of DES treatment on the viability of control and tau KD hiPSC neurons ( c ) and the rescuing effect of CPT in DES (20 mM) treated control hiPSC neurons ( d ). e - g , WB and quantification showing the effect of inhibiting VDAC, Hsp70, or Hsp90 on PHF-1 tau entry into mitochondria without affecting total PHF-1 tau levels ( e ), and quantification of the effect of inhibitor treatment on RET activity ( f ) and stress sensitivity ( g ) of APP hiPSC neurons. h , Measurement of RET ROS and NAD + /NADH in purified mitochondria from control and tau-WT-EGFP or tau-S2A-EGFP-transfected normal hiPSC neurons. i , Representative images and quantification of p-S262 tau in tau-WT-EGFP or tau-S2A-EGFP transfected control hiPSC neurons with or without DES treatment. j , Measurement of RET ROS and NAD + /NADH in purified mitochondria from EGFP or MKI-EGFP transduced APP hiPSC neurons. k , Representative images and quantification of p-S262 tau in EGFP or MKI-EGFP transfected APP hiPSC neurons. l, m , Immunoblots ( l ) and quantification ( m ) showing effects of the various treatments on normalized levels of p-tau species in APP hiPSC neurons. n, o , Immunoblots ( n ) and quantification ( o ) showing effect of DES or DES/CPT co-treatment on normalized levels of p-tau species in control hiPSC neurons. All data are means ± SEM; statistical significance was determined by one-way ANOVA with Tukey’s post hoc test ( a, b, d, g, h, i, o ), two-way ANOVA with Tukey’s post hoc test ( c ), two-tailed unpaired Student’s t test ( j, k ), or one-way ANOVA with Dunnett’s multiple test ( e, f , m ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001 ( a, b, c, d, g : n=6/group from 3 biological replicates and 2 wells/experiment; e, f, h, j, m, o : n=3 biological replicates; i, k : n=3 biological replicates, and each data point represents an average of 6 cells/experiment).

    Article Snippet: The following primary antibodies and dilutions were used: mouse anti-tau PHF1, 1:500 (Gift of Peter Davies); mouse anti-tau CP13, 1:500 (Gift of Peter Davies); rat anti-dSDHA and rabbit anti-dNDUFV1 1:1000 (Gift from Dr. Edward Owusu-Ansah); p-S262 anti-tau antibody 1:500 (ThermoFisher #44-750G); mouse anti-dTau 1:500 (DSHB #5A6); rabbit anti-Tom20 1:1000 (abclonal #A19403); rabbit anti-cytochrome c 1:1000 (Proteintech #10993-1-AP); rabbit anti-LonP1 1:1000 (Proteintech #15440-1-AP); rabbit anti-Map2 1:1000 (Proteintech #17490-1-AP); rabbit anti-NDUFS1 1:1000 (Proteintech #12444-1-AP); rabbit anti-NDUFA10 1:1000 (Proteintech #32947-1-AP); rabbit anti-SDHA 1:1000 (Proteintech #14865-1-AP), rabbit anti-ATP5α 1:1000 (abcam #ab14748); mouse anti-tau AT100, 1:500 (Invitrogen, MN1060); mouse anti-tau HT7, 1:1000 (Invitrogen, MN1000); mouse anti-Tau7, 1:1000 (Sigma, MAB2239); rabbit anti-tau Thr231, 1:500 (Invitrogen, 44-746G); rabbit anti-NDUFV1, 1:1000 (Proteintech, 11238-1-AP); rabbit anti-NDUFS3, 1:1000 (Proteintech, 15066-1-AP); rabbit anti-GFAP, 1:500 (Proteintech, 16825-1-AP); rabbit anti-IL-1β, 1:500 (Proteintech, 16806-1-AP); rabbit anti-Cleaved Caspase-1, 1:500 (Cell Signaling Technology, 4199T); rabbit anti-ASC/TMS1, 1:500 (Cell Signaling Technology, 67824T); rabbit anti-Drp1, 1:500 (Cell Signaling Technology, 4494S); rabbit anti-Opa1, 1:500 (Cell Signaling Technology, 80471T); rabbit anti-Glu2B, 1:500 (Cell Signaling Technology, 4212T); rabbit anti-p-GSK3β, 1:500 (Cell Signaling Technology, 5558T); rabbit anti-GSK3β, 1:500 (Cell Signaling Technology, 12456S); rabbit anti-MARK4, 1:500 (Cell Signaling Technology, 4834S); rabbit anti-MFN2, 1:500 (ProSci, 7863); mouse anti-PSD95, 1:500 (BioLegend, 810302); mouse anti-PP2A, 1:500 (BD Biosciences, 610556); mouse anti-Tau46, 1:500 (Sigma, T9450); rabbit anti-p-PAR-1(T408) , 1:500; rabbit anti-VDAC1, 1:500 (Proteintech, 55259-1-AP); rabbit anti-Hsp70, 1:200 (Santa Cruze, sc-25837); rabbit anti-Hsp90, 1:500 (Proteintech, 13171-1-AP).

    Techniques: Control, Activity Assay, Purification, Transfection, Western Blot, Two Tailed Test